Jafree, DJ;
Long, DA;
Scambler, PJ;
Moulding, D;
(2019)
Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy.
Methods in Molecular Biology
, 2067
pp. 103-126.
10.1007/978-1-4939-9841-8_8.
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Abstract
Microscopic and macroscopic evaluation of biological tissues in three dimensions is becoming increasingly popular. This trend is coincident with the emergence of numerous tissue clearing strategies, and advancements in confocal and two-photon microscopy, enabling the study of intact organs and systems down to cellular and sub-cellular resolution. In this chapter, we describe a wholemount immunofluorescence technique for labeling structures in renal tissue. This technique combined with solvent-based tissue clearing and confocal imaging, with or without two-photon excitation, provides greater structural information than traditional sectioning and staining alone. Given the addition of paraffin embedding to our method, this hybrid protocol offers a powerful approach to combine confocal or two-photon findings with histological and further immunofluorescent analysis within the same tissue.
| Type: | Article |
|---|---|
| Title: | Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-9841-8_8 |
| Publisher version: | https://doi.org/10.1007/978-1-4939-9841-8_8 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Confocal, Imaging, Kidney, Three-dimensional, Tissue clearing, Two-photon |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10093852 |
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