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Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals

King, CM; Bohmbach, K; Minge, D; Delekate, A; Zheng, K; Reynolds, J; Rakers, C; ... Henneberger, C; + view all (2020) Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals. Cell Reports , 30 (10) 3466-3477.e4. 10.1016/j.celrep.2020.02.043. Green open access

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Abstract

Astroglia regulate neurovascular coupling while engaging in signal exchange with neurons. The underlying cellular machinery is thought to rely on astrocytic Ca2+ signals, but what controls their amplitude and waveform is poorly understood. Here, we employ time-resolved two-photon excitation fluorescence imaging in acute hippocampal slices and in cortex in vivo to find that resting [Ca2+] predicts the scale (amplitude) and the maximum (peak) of astroglial Ca2+ elevations. We bidirectionally manipulate resting [Ca2+] by uncaging intracellular Ca2+ or Ca2+ buffers and use ratiometric imaging of a genetically encoded Ca2+ indicator to establish that alterations in resting [Ca2+] change co-directionally the peak level and anti-directionally the amplitude of local Ca2+ transients. This relationship holds for spontaneous and for induced (for instance by locomotion) Ca2+ signals. Our findings uncover a basic generic rule of Ca2+ signal formation in astrocytes, thus also associating the resting Ca2+ level with the physiological “excitability” state of astroglia.

Type: Article
Title: Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.celrep.2020.02.043
Publisher version: https://doi.org/10.1016/j.celrep.2020.02.043
Language: English
Additional information: © 2020 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
Keywords: astrocytes, brain, calcium signalling, calcium stores, cortex, fluorescence microscopy, hippocampus, in vivo, locomotion, mechanism, quantitative
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy
URI: https://discovery.ucl.ac.uk/id/eprint/10093561
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