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Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans

Doitsidou, M; Jarriault, S; Poole, RJ; (2016) Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans. Genetics , 204 (2) pp. 451-474. 10.1534/genetics.115.186197. Green open access

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Abstract

The use of next-generation sequencing (NGS) has revolutionized the way phenotypic traits are assigned to genes. In this review, we describe NGS-based methods for mapping a mutation and identifying its molecular identity, with an emphasis on applications in Caenorhabditis elegans. In addition to an overview of the general principles and concepts, we discuss the main methods, provide practical and conceptual pointers, and guide the reader in the types of bioinformatics analyses that are required. Owing to the speed and the plummeting costs of NGS-based methods, mapping and cloning a mutation of interest has become straightforward, quick, and relatively easy. Removing this bottleneck previously associated with forward genetic screens has significantly advanced the use of genetics to probe fundamental biological processes in an unbiased manner.

Type: Article
Title: Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans
Open access status: An open access version is available from UCL Discovery
DOI: 10.1534/genetics.115.186197
Publisher version: https://doi.org/10.1534/genetics.115.186197
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisherís terms and conditions.
Keywords: WormBook, mapping-by-sequencing, next-generation sequencing, positional cloning, mutation identification, Caenorhabditis elegans,
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10093462
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