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High-Throughput Automated Olfactory Phenotyping of Group-Housed Mice

Reinert, JK; Schaefer, AT; Kuner, T; (2019) High-Throughput Automated Olfactory Phenotyping of Group-Housed Mice. Frontiers in Behavioral Neuroscience , 13 , Article 267. 10.3389/fnbeh.2019.00267. Green open access

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Abstract

Behavioral phenotyping of mice is often compromised by manual interventions of the experimenter and limited throughput. Here, we describe a fully automated behavior setup that allows for quantitative analysis of mouse olfaction with minimized experimenter involvement. Mice are group-housed and tagged with unique RFID chips. They can freely initiate trials and are automatically trained on a go/no-go task, learning to distinguish a rewarded from an unrewarded odor. Further, odor discrimination tasks and detailed training aspects can be set for each animal individually for automated execution without direct experimenter intervention. The procedure described here, from initial RFID implantation to discrimination of complex odor mixtures at high accuracy, can be completed within <2 months with cohorts of up to 25 male mice. Apart from the presentation of monomolecular odors, the setup can generate arbitrary mixtures and dilutions from any set of odors to create complex stimuli, enabling demanding behavioral analyses at high-throughput.

Type: Article
Title: High-Throughput Automated Olfactory Phenotyping of Group-Housed Mice
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fnbeh.2019.00267
Publisher version: https://doi.org/10.3389/fnbeh.2019.00267
Language: English
Additional information: © 2019 Reinert, Schaefer and Kuner. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
Keywords: olfaction, operant conditioning, automated, behavior assay, olfactory testing
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/10093458
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