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Modulation of neuronal cell affinity of composites scaffolds based on polyhydroxyalkanoates and bioactive glasses

Lizarraga Valderrama, LDR; Nigmatullin, R; Ladino, B; Taylor, CS; Boccaccini, AR; Knowles, JC; Claeyssens, F; ... Roy, I; + view all (2020) Modulation of neuronal cell affinity of composites scaffolds based on polyhydroxyalkanoates and bioactive glasses. Biomedical Materials 10.1088/1748-605X/ab797b. (In press). Green open access

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Abstract

Biocompatibility and neuron regenerating properties of various bioactive glass (BG)/Polyhydroxyalkanoate (PHA) blend composites were assessed in order to study their suitability for peripheral nerve tissue applications, specifically as lumen structures for nerve guidance conduits (NGCs). BG/PHA blend composites were fabricated using Bioactive glass® 45S5 (BG1) and BG 1393 (BG2) with the 25:35 poly(3-hydroxyoctanoate/poly3-hydroxybutyrate), 25:75 P(3HO)/P(3HB) blend (PHA blend). Various concentrations of each BG (0.5, 1.0 and 2.5 wt%) were used to determine the effect of BG on neuronal growth and differentiation, in single culture using NG108-15 neuronal cells and in a co-culture along with RN22 Schwann cells. NG108-15 cells exhibited good growth and differentiation on all the PHA blend composites showing that both BGs have good biocompatibility at 0.5, 1.0 and 2.5 wt% within the PHA blend. The Young's modulus values displayed by all the PHA blend/BG composites ranged from 385.6 MPa to 1792.6 MPa, which are able to provide the required support and protective effect for regeneration of peripheral nerves. More specifically, the tensile strength obtained in the PHA blend/BG1 (1.0 wt%) (10.0 ± 0.6 MPa) was found to be similar to that of rabbit peroneal nerve. This composite also exhibited the best biological performance in supporting growth and neuronal differentiation among all the substrates. The neurite extension on this composite was found to be remarkable with the neurites forming a complex connection network.

Type: Article
Title: Modulation of neuronal cell affinity of composites scaffolds based on polyhydroxyalkanoates and bioactive glasses
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1088/1748-605X/ab797b
Publisher version: https://doi.org/10.1088/1748-605X/ab797b
Language: English
Additional information: As the Version of Record of this article is going to be/has been published on a gold open access basis under a CC BY 3.0 licence, this Accepted Manuscript is available for reuse under a CC BY 3.0 licence immediately (https://creativecommons.org/licenses/by/3.0/). Although reasonable endeavours have been taken to obtain all necessary permissions from third parties to include their copyrighted content within this article, their full citation and copyright line may not be present in this Accepted Manuscript version. Before using any content from this article, please refer to the Version of Record on IOPscience once published for full citation and copyright details, as permission may be required. All third party content is fully copyright protected, and is not published on a gold open access basis under a CC BY licence, unless that is specifically stated in the figure caption in the Version of Record.
Keywords: nerve regeneration, nerve tissue engineering, bioactive glasses, polyhydroxyalkanoates, composite scaffolds
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10093111
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