Joubert, R;
Mariot, V;
Dumonceaux, J;
(2020)
A one-hour universal protocol for mouse genotyping.
Muscle and Nerve
, 61
(6)
pp. 801-807.
10.1002/mus.26841.
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Abstract
Introduction Transgenic animals are widely used for research and for most of them, genotyping is unavoidable. Published protocols may be powerful but may also present disadvantages such as their cost or the requirement of additional steps/equipment. Moreover, if more than one strain must be genotyped, several protocols may need to be developed. Methods we adapted the existing amplification‐resistant mutation protocol to develop the 1‐hour universal genotyping protocol (1‐HUG), which allows the robust genotyping of genetically modified mice in 1 h from sample isolation to PCR gel running. Results This protocol allows the genotyping of different mouse models including mdx mouse, and FLExDUX4 and HSA‐MerCreMer alone or in combination. It can be applied to different types of genomic modifications and to sexing. Discussion The 1‐HUG protocol can be used routinely in any laboratory using mouse models for neuromuscular diseases.
| Type: | Article |
|---|---|
| Title: | A one-hour universal protocol for mouse genotyping |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/mus.26841 |
| Publisher version: | https://doi.org/10.1002/mus.26841 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Genotyping, mouse model, FSHD, DMD, neuromuscular diseases |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Neurosciences Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10092015 |
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