Takeuchi, Y;
Narumi, R;
Akiyama, R;
Vitiello, E;
Shirai, T;
Tanimura, N;
Kuromiya, K;
... Fujita, Y; + view all
(2020)
Calcium Wave Promotes Cell Extrusion.
Current Biology
, 30
(4)
670-681.e6.
10.1016/j.cub.2019.11.089.
Preview |
Text
Tada_Takeuchi et al 2020-op.pdf - Accepted Version Download (9MB) | Preview |
Abstract
When oncogenic transformation or apoptosis occurs within epithelia, the harmful or dead cells are apically extruded from tissues to maintain epithelial homeostasis. However, the underlying molecular mechanism still remains elusive. In this study, we first show, using mammalian cultured epithelial cells and zebrafish embryos, that prior to apical extrusion of RasV12-transformed cells, calcium wave occurs from the transformed cell and propagates across the surrounding cells. The calcium wave then triggers and facilitates the process of extrusion. IP3 receptor, gap junction, and mechanosensitive calcium channel TRPC1 are involved in calcium wave. Calcium wave induces the polarized movement of the surrounding cells toward the extruding transformed cells. Furthermore, calcium wave facilitates apical extrusion, at least partly, by inducing actin rearrangement in the surrounding cells. Moreover, comparable calcium propagation also promotes apical extrusion of apoptotic cells. Thus, calcium wave is an evolutionarily conserved, general regulatory mechanism of cell extrusion.
| Type: | Article |
|---|---|
| Title: | Calcium Wave Promotes Cell Extrusion |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.cub.2019.11.089 |
| Publisher version: | https://doi.org/10.1016/j.cub.2019.11.089 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Calcium wave; epithelial homeostasis; cell extrusion; RasV12-transformed; apoptosis; actin rearrangement; TRPC1; INF2 |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10091902 |
Archive Staff Only
 |
View Item |
