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Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters

Dergai, O; Cousin, P; Gouge, J; Satia, K; Praz, V; Kuhlman, T; Lhôte, P; ... Hernandez, N; + view all (2018) Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters. Genes and Development , 32 (9-10) pp. 711-722. 10.1101/gad.314245.118. Green open access

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Abstract

RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the SNAP complex (SNAPc). The main distinguishing feature is the presence, in the type 3 promoters only, of a TATA box, which determines Pol III specificity. To understand the mechanism by which the absence or presence of a TATA box results in specific Pol recruitment, we examined how SNAPc and general transcription factors required for Pol II or Pol III transcription of SNAPc-dependent genes (i.e., TATA-box-binding protein [TBP], TFIIB, and TFIIA for Pol II transcription and TBP and BRF2 for Pol III transcription) assemble to ensure specific Pol recruitment. TFIIB and BRF2 could each, in a mutually exclusive fashion, be recruited to SNAPc. In contrast, TBP-TFIIB and TBP-BRF2 complexes were not recruited unless a TATA box was present, which allowed selective and efficient recruitment of the TBP-BRF2 complex. Thus, TBP both prevented BRF2 recruitment to Pol II promoters and enhanced BRF2 recruitment to Pol III promoters. On Pol II promoters, TBP recruitment was separate from TFIIB recruitment and enhanced by TFIIA. Our results provide a model for specific Pol recruitment at SNAPc-dependent promoters.

Type: Article
Title: Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1101/gad.314245.118
Publisher version: https://doi.org/10.1101/gad.314245.118
Language: English
Additional information: This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
Keywords: BRF2, SNAPc, TBP, TFIIA, TFIIB, small nuclear RNA promoters, HEK293 Cells, Humans, Mutation, Promoter Regions, Genetic, Protein Binding, Protein Domains, Protein Transport, RNA Polymerase II, RNA Polymerase III, RNA, Small Nuclear, TATA Box, TATA-Box Binding Protein, Transcription Factor TFIIB, Transcription Factors
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10091773
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