UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Determinants of Polar versus Nematic Organization in Networks of Dynamic Microtubules and Mitotic Motors

Roostalu, J; Rickman, J; Thomas, C; Nédélec, F; Surrey, T; (2018) Determinants of Polar versus Nematic Organization in Networks of Dynamic Microtubules and Mitotic Motors. Cell , 175 (3) 796-808.e14. 10.1016/j.cell.2018.09.029. Green open access

[img]
Preview
Text
Roostalu_Rickman_Cell.pdf - Published version

Download (11MB) | Preview

Abstract

During cell division, mitotic motors organize microtubules in the bipolar spindle into either polar arrays at the spindle poles or a “nematic” network of aligned microtubules at the spindle center. The reasons for the distinct self-organizing capacities of dynamic microtubules and different motors are not understood. Using in vitro reconstitution experiments and computer simulations, we show that the human mitotic motors kinesin-5 KIF11 and kinesin-14 HSET, despite opposite directionalities, can both organize dynamic microtubules into either polar or nematic networks. We show that in addition to the motor properties the natural asymmetry between microtubule plus- and minus-end growth critically contributes to the organizational potential of the motors. We identify two control parameters that capture system composition and kinetic properties and predict the outcome of microtubule network organization. These results elucidate a fundamental design principle of spindle bipolarity and establish general rules for active filament network organization.

Type: Article
Title: Determinants of Polar versus Nematic Organization in Networks of Dynamic Microtubules and Mitotic Motors
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.cell.2018.09.029
Publisher version: https://doi.org/10.1016/j.cell.2018.09.029
Language: English
Additional information: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: microtubule, cytoskeleton, kinesin, molecular motor, spindle assembly, self-organization, active network, in vitro reconsititution, computer simulation, Cytosim
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of the Built Environment
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of the Built Environment > Bartlett School Env, Energy and Resources
URI: https://discovery.ucl.ac.uk/id/eprint/10091332
Downloads since deposit
4Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item