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Discovery of a novel fluorescent chemical probe suitable for evaluation of neuropilin‐1 binding of small molecules

Conole, D; Chou, Y-T; Patsiarika, A; Nwabo, V; Dinnitriou, E; Soudy, C; Mota, F; ... Selwood, DL; + view all (2020) Discovery of a novel fluorescent chemical probe suitable for evaluation of neuropilin‐1 binding of small molecules. Drug Development Research 10.1002/ddr.21641. (In press). Green open access

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Abstract

Neuropilin‐1 (NRP1) is emerging as an important molecule in immune signaling where it has been shown to modulate the actions of TGF‐β1 in macrophages and regulatory T cells. The development of cost‐effective and reliable assays for NRP1 binding is therefore important. We synthesized three new NRP1 small molecule fluorophores and examined their performance as fluorescent polarization probes. One molecule DS108 exhibited favorable binding and fluorescent characteristics and allowed us to establish a simple assay suitable for medium to high throughput screening of small molecules.

Type: Article
Title: Discovery of a novel fluorescent chemical probe suitable for evaluation of neuropilin‐1 binding of small molecules
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/ddr.21641
Publisher version: https://doi.org/10.1002/ddr.21641
Language: English
Additional information: This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: fluorescence polarization, neuropilin-1, surface plasmon resonance
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research
URI: https://discovery.ucl.ac.uk/id/eprint/10090938
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