Pereira, P;
Gustafsson, N;
Marsh, M;
Mhlanga, M;
Henriques, R;
(2020)
Super-Beacons: open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy.
BioRxiv: Cold Spring Harbor, NY, USA.
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Abstract
Localization based super-resolution microscopy relies on the detection of individual molecules cycling between fluorescent and non-fluorescent states. These transitions are commonly regulated by high-intensity illumination, imposing constrains to imaging hardware and producing sample photodamage. Here, we propose single-molecule self-quenching as a mechanism to generate spontaneous photoswitching independent of illumination. To demonstrate this principle, we developed a new class of DNA-based open-source Super-Resolution probes named Super-Beacons, with photoswitching kinetics that can be tuned structurally, thermally and chemically. The potential of these probes for live-cell friendly Super-Resolution Microscopy without high-illumination or toxic imaging buffers is revealed by imaging Interferon Inducible Transmembrane proteins (IFITMs) at sub-100nm resolutions.
| Type: | Working / discussion paper |
|---|---|
| Title: | Super-Beacons: open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1101/2020.01.20.912311 |
| Publisher version: | https://doi.org/10.1101/2020.01.20.912311 |
| Language: | English |
| Additional information: | The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license (http://creativecommons.org/licenses/by/4.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10090448 |
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