UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection

Sousa-Nunes, R; Rana, AA; Kettleborough, R; Brickman, JM; Clements, M; Forrest, A; Grimmond, S; ... Beddington, RS; + view all (2003) Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection. Genome Research , 13 (12) pp. 2609-2620. 10.1101/gr.1362303. Green open access

[thumbnail of Genome Res.-2003-Sousa-Nunes-2609-20.pdf]
Preview
Text
Genome Res.-2003-Sousa-Nunes-2609-20.pdf - Published version

Download (1MB) | Preview

Abstract

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node–tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

Type: Article
Title: Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection
Open access status: An open access version is available from UCL Discovery
DOI: 10.1101/gr.1362303
Publisher version: https://doi.org/10.1101/gr.1362303
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
URI: https://discovery.ucl.ac.uk/id/eprint/10090342
Downloads since deposit
24Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item