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Caspase 3/ROCK1 pathway mediates high glucose-induced platelet microparticles shedding

Wang, GH; Ma, KL; Zhang, Y; Hu, ZB; Liu, L; Lu, J; Chen, PP; ... Liu, BC; + view all (2019) Caspase 3/ROCK1 pathway mediates high glucose-induced platelet microparticles shedding. Biochemical and Biophysical Research Communications , 509 (2) pp. 596-602. 10.1016/j.bbrc.2018.12.166. Green open access

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Abstract

Background: Platelet microparticles (PMPs) are closely associated with diabetic macrovascular complications. This study aimed to explore the underlying mechanisms of high glucose-induced PMPs generation. Methods: Washed platelets, obtained from the plasma of healthy male Sprague-Dawley rats, were incubated with high glucose. PMPs were isolated using gradient centrifugation and counted by flow cytometry. Expression and activity of ROCK1 and caspase3 were evaluated by real-time PCR, Western blotting, and activity assay kit. Results: High glucose enhanced PMPs shedding in the presence of collagen. The mRNA and protein levels of ROCK1, but not ROCK2, were increased in platelets incubated with high glucose. Y-27632, an inhibitor of ROCK, blocked the increased PMPs shedding induced by high glucose. Expression and activity of caspase3 were elevated in platelets under the high glucose conditions. Z-DVED-FMK, a caspase3 inhibitor, inhibited ROCK1 activity and decreased the PMPs generation under high glucose. Conclusion: High glucose increased PMPs shedding via caspase3-ROCK1 signal pathway.

Type: Article
Title: Caspase 3/ROCK1 pathway mediates high glucose-induced platelet microparticles shedding
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bbrc.2018.12.166
Publisher version: https://doi.org/10.1016/j.bbrc.2018.12.166
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Platelet microparticles, High glucose, Rho-associated kinase 1, caspase3, ACTIVATION, THROMBIN, CYTOSKELETON, INVOLVEMENT, TYPE-1
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Renal Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10090304
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