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Continual Conscious Bioluminescent Imaging in Freely Moving Mice

Diaz, JA; Geard, A; FitzPatrick, LM; Delhove, JMKM; Buckley, SMK; Waddington, SN; McKay, TR; (2019) Continual Conscious Bioluminescent Imaging in Freely Moving Mice. Methods in Molecular Biology , 2081 pp. 161-175. 10.1007/978-1-4939-9940-8_11. Green open access

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Continual Conscious Bioluminescent Imaging in Freely Moving Mice. Methods Mol Biol , 2081 pp..pdf - Accepted version

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Abstract

In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R’s (replacement, reduction, and refinement) of biomedical animal research.

Type: Article
Title: Continual Conscious Bioluminescent Imaging in Freely Moving Mice
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-4939-9940-8_11
Publisher version: https://doi.org/10.1007/978-1-4939-9940-8_11
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Lentiviral vectors, Adeno-associated vectors, Transcription factor activated reporter, Biosensors, Luciferase, Neonatal mice, Luciferin, Bioluminescence imaging, Conscious imaging
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL EGA Institute for Womens Health > Maternal and Fetal Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10090105
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