Majeed, SR;
Vasudevan, L;
Chen, C-Y;
Luo, Y;
Torres, JA;
Evans, TM;
Sharkey, A;
... Brodsky, FM; + view all
(2014)
Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration.
Nature Communications
, 5
, Article 3891. 10.1038/ncomms4891.
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Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration.pdf - Published Version Download (5MB) | Preview |
Abstract
The clathrin light chain (CLC) subunits participate in several membrane traffic pathways involving both clathrin and actin, through binding the actin-organizing huntingtin-interacting proteins (Hip). However, CLCs are dispensable for clathrin-mediated endocytosis of many cargoes. Here we observe that CLC depletion affects cell migration through Hip binding and reduces surface expression of β1-integrin by interference with recycling following normal endocytosis of inactive β1-integrin. CLC depletion and expression of a modified CLC also inhibit the appearance of gyrating (G)-clathrin structures, known mediators of rapid recycling of transferrin receptor from endosomes. Expression of the modified CLC reduces β1-integrin and transferrin receptor recycling, as well as cell migration, implicating G-clathrin in these processes. Supporting a physiological role for CLC in migration, the CLCb isoform of CLC is upregulated in migratory human trophoblast cells during uterine invasion. Together, these studies establish CLCs as mediating clathrin–actin interactions needed for recycling by G-clathrin during migration.
| Type: | Article |
|---|---|
| Title: | Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/ncomms4891 |
| Publisher version: | https://doi.org/10.1038/ncomms4891 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/. |
| Keywords: | Cell migration, Cell signalling, Membrane trafficking, Metastasis |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10088830 |
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