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In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography

Madi, K; Staines, KA; Bay, BK; Javaheri, B; Geng, H; Bodey, AJ; Cartmell, S; ... Lee, PD; + view all (2020) In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography. Nature Biomedical Engineering , 4 pp. 343-354. 10.1038/s41551-019-0477-1. Green open access

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Abstract

Imaging techniques for quantifying changes in the hierarchical structure of deforming joints are constrained by destructive sample treatments, sample-size restrictions and lengthy scan times. Here, we report the use of fast low-dose pink-beam synchrotron X-ray tomography in combination with mechanical loading at nanometric precision for in situ imaging, at resolutions below 100 nm, of the mechanical strain in intact untreated joints under physiologically realistic conditions. We show that in young, older and osteoarthritic mice, hierarchical changes in tissue structure and mechanical behaviour can be simultaneously visualized, and that the tissue structure at the cellular level correlates with the mechanical performance of the whole joint. We also use the tomographic approach to study the colocalization of tissue strains to specific chondrocyte lacunar organizations within intact loaded joints and to explore the role of calcified-cartilage stiffness on the biomechanics of healthy and pathological joints.

Type: Article
Title: In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41551-019-0477-1
Publisher version: https://doi.org/10.1038/s41551-019-0477-1
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Biomedical engineering, Bone quality and biomechanics, Imaging techniques, Osteoarthritis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10088737
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