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Combination light-based therapies to treat pancreatic cancer: A proof of concept

Ney, A; Mahamed, I; Garcia-Sampedro, A; Selbo, PK; Sancho, P; MacRobert, AJ; Pereira, SP; (2019) Combination light-based therapies to treat pancreatic cancer: A proof of concept. In: Proceedings of the 17th International Photodynamic Association World Congress. (pp. p. 1107045). SPIE: Cambridge, MA, USA. Green open access

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Abstract

Pancreatic ductal adenocarcinoma remains one of the worst types of cancers mainly due to its late diagnosis, lack of effective therapies for advance disease and high chemoresistance. Novel therapeutic options that could improve patient quality of life and overall survival are therefore imperative. In this study, we describe the use of an original strategy based on photochemical internalisation (PCI) technology for pancreatic cancer treatment. Subcellular localisation of the photosensitiser meso-tetraphenylporphine-disulfonate (TPPS2a) was performed in PANC-1 cells, showing its preferential accumulation in lysosomes. Treatments with increasing concentrations of the ribosome-inactivating protein saporin or TPPS2a alone were compared with PCI-saporin. Metabolic activity and cell viability of PANC-1 cells were determined 96h post-illumination by MTT and trypan blue assays, respectively. Our results show that PCI using the photosensitiser TPPS2a, synergistically enhances the cytotoxic effects of saporin in PANC-1 cells and could offer more effective treatment options for pancreatic cancer.

Type: Proceedings paper
Title: Combination light-based therapies to treat pancreatic cancer: A proof of concept
Event: 17th International Photodynamic Association World Congress, 2019
Open access status: An open access version is available from UCL Discovery
DOI: 10.1117/12.2525305
Publisher version: https://doi.org/10.1117/12.2525305
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inst for Liver and Digestive Hlth
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/10088320
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