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Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Giobbe, GG; Crowley, C; Luni, C; Campinoti, S; Khedr, M; Kretzschmar, K; De Santis, MM; ... De Coppi, P; + view all (2019) Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture. Nature Communications , 10 (1) , Article 5658. 10.1038/s41467-019-13605-4. Green open access

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Abstract

Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

Type: Article
Title: Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41467-019-13605-4
Publisher version: https://doi.org/10.1038/s41467-019-13605-4
Language: English
Additional information: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Extracellular matrix, Intestinal stem cells, Tissues
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10088038
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