Wiegmann, V;
Giaka, M;
Martinez, CB;
Baganz, F;
(2019)
Towards the development of automated fed-batch cell culture processes at microscale.
Biotechniques
, 67
(5)
pp. 238-241.
10.2144/btn-2019-0063.
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Abstract
AIM: To investigate the impact of various feeding strategies on the growth and productivity of a GS-CHO cell line. METHODS: Feed additions were conducted at fixed volumes or linked to a marker such as cell growth or metabolism and added as bolus or near-continuously using the automated feeding module of the micro-Matrix (Applikon). RESULTS: The selected feeding regimens supported maximum viable cell densities of up to 1.9 × 107 cells ml−1 and final titers of up to 1.13 g l−1. Differences in growth and titer between feeding strategies were insignificant, with the exception of one feeding strategy. CONCLUSION: As the more complex feeding strategies did not create an advantage, the selection of a simple feeding strategy such as bolus or continuous addition of feed medium is preferred. METHOD SUMMARY Fed-batch is an easy and popular way to intensify cell cultivations. The time of addition and the volume that is added can be decisive in achieving optimal product titers. A micro-bioreactor has been used to investigate the effect of several feeding strategies on the growth and production kinetics of a GS-CHO cell line.
| Type: | Article |
|---|---|
| Title: | Towards the development of automated fed-batch cell culture processes at microscale |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.2144/btn-2019-0063 |
| Publisher version: | https://doi.org/10.2144/btn-2019-0063 |
| Language: | English |
| Additional information: | This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Keywords: | GS-CHO cells, automation, downscale, fed-batch, miniature shaken bioreactor |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10087695 |
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