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Towards the development of automated fed-batch cell culture processes at microscale

Wiegmann, V; Giaka, M; Martinez, CB; Baganz, F; (2019) Towards the development of automated fed-batch cell culture processes at microscale. Biotechniques , 67 (5) pp. 238-241. 10.2144/btn-2019-0063. Green open access

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Abstract

AIM: To investigate the impact of various feeding strategies on the growth and productivity of a GS-CHO cell line. METHODS: Feed additions were conducted at fixed volumes or linked to a marker such as cell growth or metabolism and added as bolus or near-continuously using the automated feeding module of the micro-Matrix (Applikon). RESULTS: The selected feeding regimens supported maximum viable cell densities of up to 1.9 × 107 cells ml−1 and final titers of up to 1.13 g l−1. Differences in growth and titer between feeding strategies were insignificant, with the exception of one feeding strategy. CONCLUSION: As the more complex feeding strategies did not create an advantage, the selection of a simple feeding strategy such as bolus or continuous addition of feed medium is preferred. METHOD SUMMARY Fed-batch is an easy and popular way to intensify cell cultivations. The time of addition and the volume that is added can be decisive in achieving optimal product titers. A micro-bioreactor has been used to investigate the effect of several feeding strategies on the growth and production kinetics of a GS-CHO cell line.

Type: Article
Title: Towards the development of automated fed-batch cell culture processes at microscale
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.2144/btn-2019-0063
Publisher version: https://doi.org/10.2144/btn-2019-0063
Language: English
Additional information: This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords: GS-CHO cells, automation, downscale, fed-batch, miniature shaken bioreactor
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10087695
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