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Association study of single nucleotide polymorphisms in IL-10 and IL-17 genes with the severity of microbial keratitis

Carnt, NA; Cipriani, V; Stapleton, FJ; Calder, V; Willcox, MD; (2019) Association study of single nucleotide polymorphisms in IL-10 and IL-17 genes with the severity of microbial keratitis. Contact Lens and Anterior Eye , 42 (6) pp. 658-661. 10.1016/j.clae.2019.06.007. Green open access

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Abstract

PURPOSE Exploratory analysis to assess the association of single nucleotide polymorphisms (SNPs) in the interleukin (IL) 10 and IL-17 genes with severity of contact lens keratitis. METHODS This was a retrospective case control study of 88 contact lens keratitis cases (25 severe) and 185 healthy contact lens wearers recruited from studies conducted at Moorfields Eye Hospital and in Australia-wide during 2003–2005. Buccal swab samples were collected on Whatman FTA cards and mailed by post for DNA extraction and SNP genotyping. IL-10 (rs1800871; rs1800896; rs1800872) and IL-17 (rs1800871; rs1800896; rs1800872) SNPs were screened by pyrosequencing. Genetic association analyses were performed via Cochran-Armitage trend tests and logistic regression models using PLINK software. RESULTS None of the SNPs tested showed evidence of association with severity of contact lens keratitis at P <  0.05. Nevertheless, minor allele G in SNP rs2397084 of the IL-17F gene was associated with increased risk of severe MK, with OR=2.1 (95% CI=0.9-4.8, P = 0.066). CONCLUSION Our study cannot exclude with confidence that genetic variation in the IL-17 F proinflammatory cytokine is associated with more severe outcomes of MK. However, there is general body of information that the IL-17 pathway is important in the mechanisms of MK. Studies with larger power and the expanded array of laboratory tools will elucidate the exact role of IL-17 in MK.

Type: Article
Title: Association study of single nucleotide polymorphisms in IL-10 and IL-17 genes with the severity of microbial keratitis
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.clae.2019.06.007
Publisher version: https://doi.org/10.1016/j.clae.2019.06.007
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Contact lens, Gene, Interleukin, Keratitis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10087057
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