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Study on mixing characteristics in shaken microwell systems

Li, Y; Ducci, A; Micheletti, M; (2020) Study on mixing characteristics in shaken microwell systems. Biochemical Engineering Journal , 153 , Article 107392. 10.1016/j.bej.2019.107392. Green open access

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Abstract

Shaken microwell plates are widely used for early bioprocess development as they allow a large number of experiments to be performed in parallel by using small amount of materials. Despite their widespread use, microwell plates have not been characterised from an engineering viewpoint. In this study, mixing time measurements were carried out in two wells of square and cylindrical cross sections for small orbital diameter shaker, do = 3 mm, commonly used in commercial microwell platforms (i.e. ThermoMixer) and compared against measurements obtained in lab scale reactors for larger orbital diameters. The Dual Indicator System for Mixing Time (DISMT) method was employed for all the operating conditions investigated, and a range of rotational speeds was identified where mixing is less effective due to reduced free surface oscillation. An effective scaling parameter between microwell platforms and lab scale reactors was identified based on the natural frequency of the system, which depends only on fill volume, size and cross section of the reactor.

Type: Article
Title: Study on mixing characteristics in shaken microwell systems
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bej.2019.107392
Publisher version: https://doi.org/10.1016/j.bej.2019.107392
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Mixing, Orbitally shaken reactors, Mixing time, Microwells, Dual indicator method, Scaling
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10086683
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