Li, Y;
Ducci, A;
Micheletti, M;
(2020)
Study on mixing characteristics in shaken microwell systems.
Biochemical Engineering Journal
, 153
, Article 107392. 10.1016/j.bej.2019.107392.
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Abstract
Shaken microwell plates are widely used for early bioprocess development as they allow a large number of experiments to be performed in parallel by using small amount of materials. Despite their widespread use, microwell plates have not been characterised from an engineering viewpoint. In this study, mixing time measurements were carried out in two wells of square and cylindrical cross sections for small orbital diameter shaker, do = 3 mm, commonly used in commercial microwell platforms (i.e. ThermoMixer) and compared against measurements obtained in lab scale reactors for larger orbital diameters. The Dual Indicator System for Mixing Time (DISMT) method was employed for all the operating conditions investigated, and a range of rotational speeds was identified where mixing is less effective due to reduced free surface oscillation. An effective scaling parameter between microwell platforms and lab scale reactors was identified based on the natural frequency of the system, which depends only on fill volume, size and cross section of the reactor.
| Type: | Article |
|---|---|
| Title: | Study on mixing characteristics in shaken microwell systems |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.bej.2019.107392 |
| Publisher version: | https://doi.org/10.1016/j.bej.2019.107392 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Mixing, Orbitally shaken reactors, Mixing time, Microwells, Dual indicator method, Scaling |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10086683 |
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