Rutter, JW;
Ozdemir, T;
Galimov, ER;
Quintaneiro, LM;
Rosa, L;
Thomas, GM;
Cabreiro, F;
(2019)
Detecting Changes in the Caenorhabditis elegans Intestinal Environment Using an Engineered Bacterial Biosensor.
ACS Synthetic Biology
10.1021/acssynbio.9b00166.
(In press).
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Abstract
Caenorhabditis elegans has become a key model organism within biology. In particular, the transparent gut, rapid growing time, and ability to create a defined gut microbiota make it an ideal candidate organism for understanding and engineering the host microbiota. Here we present the development of an experimental model that can be used to characterize whole-cell bacterial biosensors in vivo. A dual-plasmid sensor system responding to isopropyl β-d-1-thiogalactopyranoside was developed and fully characterized in vitro. Subsequently, we show that the sensor was capable of detecting and reporting on changes in the intestinal environment of C. elegans after introducing an exogenous inducer into the environment. The protocols presented here may be used to aid the rational design of engineered bacterial circuits, primarily for diagnostic applications. In addition, the model system may serve to reduce the use of current animal models and aid in the exploration of complex questions within general nematode and host-microbe biology.
Type: | Article |
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Title: | Detecting Changes in the Caenorhabditis elegans Intestinal Environment Using an Engineered Bacterial Biosensor |
Location: | United States |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1021/acssynbio.9b00166 |
Publisher version: | https://doi.org/10.1021/acssynbio.9b00166 |
Language: | English |
Additional information: | © 2019 American Chemical Society. ACS Editors' Choice with CC-BY license (https://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html). |
Keywords: | Caenorhabditis elegans, E. coli Nissle 1917, biosensors, microbiome engineering, synthetic biology |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
URI: | https://discovery.ucl.ac.uk/id/eprint/10086229 |
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