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Quantifying the binding between proteins and open chromatin-like DNA sequences with gold nanorods

Pallares, RM; Thanh, NTK; Su, X; (2019) Quantifying the binding between proteins and open chromatin-like DNA sequences with gold nanorods. Chemical Communications 10.1039/c9cc07511a. (In press). Green open access

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Abstract

The binding of transcription factors to DNA is one of the main mechanisms in gene regulation. While transcription factors frequently bind to unwrapped long DNA sequences known as open chromatin structures, most bioassays that study protein–DNA binding rely on short oligonucleotide probes. In this work, we develop a gold nanorod-based colorimetric assay for the binding of transcription factors to DNA in long open chromatin-like structures. After the determination of the binding affinity and stoichiometry, we explored the effect of the probe length on the assay performance and compared it to other established techniques.

Type: Article
Title: Quantifying the binding between proteins and open chromatin-like DNA sequences with gold nanorods
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c9cc07511a
Publisher version: https://doi.org/10.1039/c9cc07511a
Language: English
Additional information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. http://creativecommons.org/licenses/by/3.0/
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
URI: https://discovery.ucl.ac.uk/id/eprint/10085727
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