UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Applications of off-gas mass spectrometry in fed-batch mammalian cell culture

Goh, H-Y; Sulu, M; Alosert, H; Lewis, GL; Josland, GD; Merriman, DE; (2019) Applications of off-gas mass spectrometry in fed-batch mammalian cell culture. Bioprocess and Biosystems Engineering 10.1007/s00449-019-02242-2. (In press). Green open access

[thumbnail of Goh2019_Article_ApplicationsOfOff-gasMassSpect.pdf]
Preview
Text
Goh2019_Article_ApplicationsOfOff-gasMassSpect.pdf - Published Version

Download (1MB) | Preview

Abstract

Off-gas analysis using a magnetic sector mass spectrometer was performed in mammalian cell cultures in the fed-batch mode at the 5 L bench and 50 L pilot scales. Factors affecting the MS gas traces were identified during the duration of the fed-batch cultures. Correlation between viable cell concentration (VCC) and oxygen concentration of the inlet gas into the bioreactor (O2-in) resulted in R2 ≈ 0.9; O2-in could be used as a proxy for VCC. Oxygen mass transfer (kLa) was also quantified throughout the culture period with antifoam addition at different time points which is shown to lower the kLa. Real-time specific oxygen consumption rate (qO2) of 2–20 pmol/cell/day throughout the bioreactor runs were within the range of values reported in literature for mammalian cell cultures. We also report, to our knowledge, the first instance of a distinct correlation between respiration quotient (RQ) and the metabolic state of the cell culture with regard to lactate production phase (average RQ > 1) and consumption phase (average RQ < 1).

Type: Article
Title: Applications of off-gas mass spectrometry in fed-batch mammalian cell culture
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s00449-019-02242-2
Publisher version: https://doi.org/10.1007/s00449-019-02242-2
Language: English
Additional information: Copyright © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Keywords: Bioprocessing, Mass spectrometry, On-line monitoring, Process analytics, Process control
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10085659
Downloads since deposit
53Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item