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Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension

Biancospino, M; Buel, GR; Niño, CA; Maspero, E; di Perrotolo, RS; Raimondi, A; Redlingshöfer, L; ... Polo, S; + view all (2019) Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension. Nature Communications , 10 (1) , Article 4974. 10.1038/s41467-019-12855-6. Green open access

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Abstract

Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively in highly polarized tissues. Using genetically-reconstituted Caco-2 cysts as proxy for polarized epithelia, we provide evidence for coordinated action of myosin VI and CLCa at the apical surface where these proteins are essential for fission of clathrin-coated pits. We further find that myosin VI and Huntingtin-interacting protein 1-related protein (Hip1R) are mutually exclusive interactors with CLCa, and suggest a model for the sequential function of myosin VI and Hip1R in actin-mediated clathrin-coated vesicle budding.

Type: Article
Title: Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41467-019-12855-6
Publisher version: https://doi.org/10.1038/s41467-019-12855-6
Language: English
Additional information: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Biochemical assays, Endocytosis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10085550
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