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A microfluidic platform culturing two cell lines paralleled under in-vivo like fluidic microenvironment for testing the tumor targeting of nanoparticles

Wei, J; Cheng, L; Li, J; Liu, Y; Yin, S; Xu, B; Wang, D; ... Liu, C; + view all (2020) A microfluidic platform culturing two cell lines paralleled under in-vivo like fluidic microenvironment for testing the tumor targeting of nanoparticles. Talanta , 208 , Article 120355. 10.1016/j.talanta.2019.120355. Green open access

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Liu_A microfluidic platform culturing two cell lines paralleled under in-vivo like fluidic microenvironment for testing the tumor targeting of nanoparticles.pdf - Accepted version

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Abstract

Nanoparticles are attractive in medicine because their surfaces can be chemically modified for targeting specific disease cells, especially for cancer. Providing an in-vivo like platform is crucial to evaluate the biological behaviours of nanoparticles. This paper presents a microfluidic device that could culture two cell lines in parallel in in-vivo like fluidic microenvironments and be used for testing the tumor targeting of folic acid - cholesterol - chitosan (FACC) nanoparticles. The uniformity and uniformity of flow fields inside the cell culture units are investigated using the finite element method and particle tracking technology. HeLa and A549 cells are cultured in the microfluidic chip under continuous media supplementation, mimicking the fluid microenvironment in vivo. Cell introducing processes are presented by the flow behaviours of inks with different colours. The two cell lines are identified by detecting folate receptors on the cellular membranes. The growth curves of the two cell lines are measured. The two cell lines cultured paralleled inside the microfluidic device are treated with FITC-FACC to investigate the targeting of FACC. The tumor targeting of FACC are also detected by in vivo imaging of HeLa cells growth in nude mice models. The results indicate that the microfluidic device could provide a dynamic, uniform and stable fluidic microenvironment to test the tumor targeting of FACC nanoparticles.

Type: Article
Title: A microfluidic platform culturing two cell lines paralleled under in-vivo like fluidic microenvironment for testing the tumor targeting of nanoparticles
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.talanta.2019.120355
Publisher version: https://doi.org/10.1016/j.talanta.2019.120355
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Microfluidic, Paralleled introducing, Microenvironment, Targeting, Nanoparticles
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10084811
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