Dragojević, T;
Vidal Rosas, EE;
Hollmann, JL;
Culver, JP;
Justicia, C;
Durduran, T;
(2019)
High-density speckle contrast optical tomography of cerebral blood flow response to functional stimuli in the rodent brain.
Neurophotonics
, 6
(4)
, Article 045001. 10.1117/1.NPh.6.4.045001.
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Abstract
Noninvasive, three-dimensional, and longitudinal imaging of cerebral blood flow (CBF) in small animal models and ultimately in humans has implications for fundamental research and clinical applications. It enables the study of phenomena such as brain development and learning and the effects of pathologies, with a clear vision for translation to humans. Speckle contrast optical tomography (SCOT) is an emerging optical method that aims to achieve this goal by directly measuring three-dimensional blood flow maps in deep tissue with a relatively inexpensive and simple system. High-density SCOT is developed to follow CBF changes in response to somatosensory cortex stimulation. Measurements are carried out through the intact skull on the rat brain. SCOT is able to follow individual trials in each brain hemisphere, where signal averaging resulted in comparable, cortical images to those of functional magnetic resonance images in spatial extent, location, and depth. Sham stimuli are utilized to demonstrate that the observed response is indeed due to local changes in the brain induced by forepaw stimulation. In developing and demonstrating the method, algorithms and analysis methods are developed. The results pave the way for longitudinal, nondestructive imaging in preclinical rodent models that can readily be translated to the human brain.
| Type: | Article |
|---|---|
| Title: | High-density speckle contrast optical tomography of cerebral blood flow response to functional stimuli in the rodent brain |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1117/1.NPh.6.4.045001 |
| Publisher version: | https://doi.org/10.1117/1.NPh.6.4.045001 |
| Language: | English |
| Additional information: | Copyright © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
| Keywords: | blood or tissue constituent monitoring, functional monitoring and imaging, medical and biological imaging, speckle imaging |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10084671 |
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