Hogan, T;
Yates, A;
Seddon, B;
(2017)
Generation of Busulfan Chimeric Mice for the Analysis of T Cell Population Dynamics.
Bio-Protocol
, 7
(24)
, Article e2650. 10.21769/bioprotoc.2650.
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Abstract
This protocol was developed to generate chimeric mice in which T lymphocytes could be stratified by age on the basis of congenic marker expression. The conditioning drug busulfan is used to ablate host haematopoietic stem cells while leaving the peripheral immune system intact. Busulfan treatment is followed by bone marrow transplantation (BMT), with T-cell depleted donor bone marrow bearing a different congenic marker (CD45.2) to that of the host mouse (CD45.1). New cell production post-BMT can thus be tracked by measuring the fraction of CD45.2^{+} cells over time within a population of interest (Hogan et al., 2015; Gossel et al., 2017).
| Type: | Article |
|---|---|
| Title: | Generation of Busulfan Chimeric Mice for the Analysis of T Cell Population Dynamics |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.21769/bioprotoc.2650 |
| Publisher version: | https://doi.org/10.21769/BioProtoc.2650 |
| Language: | English |
| Additional information: | © Hogan et al. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | T cells, T cell homeostasis, Bone marrow chimeras, Busulfan, Temporal fate mapping |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10083840 |
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