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Design and Use of de novo Cascades for the Biosynthesis of New Benzylisoquinoline Alkaloids

Wang, Y; Tappertzhofen, N; Méndez‐Sánchez, D; Bawn, M; Lyu, B; Ward, JM; Hailes, HC; (2019) Design and Use of de novo Cascades for the Biosynthesis of New Benzylisoquinoline Alkaloids. Angewandte Chemie , 131 (30) pp. 10226-10231. 10.1002/ange.201902761. Green open access

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Abstract

The benzylisoquinoline alkaloids (BIAs) are an important group of secondary metabolites from higher plants and have been reported to show significant biological activities. The production of BIAs through synthetic biology approaches provides a higher‐yielding strategy than traditional synthetic methods or isolation from plant material. However, the reconstruction of BIA pathways in microorganisms by combining heterologous enzymes can also give access to BIAs through cascade reactions. Most importantly, non‐natural BIAs can be generated through such artificial pathways. In the current study, we describe the use of tyrosinases and decarboxylases and combine these with a transaminase enzyme and norcoclaurine synthase for the efficient synthesis of several BIAs, including six non‐natural alkaloids, in cascades from l‐tyrosine and analogues.

Type: Article
Title: Design and Use of de novo Cascades for the Biosynthesis of New Benzylisoquinoline Alkaloids
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/ange.201902761
Publisher version: https://doi.org/10.1002/ange.201902761
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Alkaloide, Biokatalyse, Enzymkaskaden, Tyrosinase, Tyrosin-Decarboxylase
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10083522
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