Nguyen, HP;
Stewart, S;
Kukwikila, MN;
Jones, SF;
Offenbartl‐Stiegert, D;
Mao, S;
Balasubramanian, S;
... Howorka, S; + view all
(2019)
A Photo‐responsive Small‐Molecule Approach for the Opto‐epigenetic Modulation of DNA Methylation.
Angewandte Chemie
, 131
(20)
pp. 6692-6696.
10.1002/ange.201901139.
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Abstract
Controlling the functional dynamics of DNA within living cells is essential in biomedical research. Epigenetic modifications such as DNA methylation play a key role in this endeavour. DNA methylation can be controlled by genetic means. Yet there are few chemical tools available for the spatial and temporal modulation of this modification. Herein, we present a small‐molecule approach to modulate DNA methylation with light. The strategy uses a photo‐tuneable version of a clinically used drug (5‐aza‐2′‐deoxycytidine) to alter the catalytic activity of DNA methyltransferases, the enzymes that methylate DNA. After uptake by cells, the photo‐regulated molecule can be light‐controlled to reduce genome‐wide DNA methylation levels in proliferating cells. The chemical tool complements genetic, biochemical, and pharmacological approaches to study the role of DNA methylation in biology and medicine.
Type: | Article |
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Title: | A Photo‐responsive Small‐Molecule Approach for the Opto‐epigenetic Modulation of DNA Methylation |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1002/ange.201901139 |
Publisher version: | https://doi.org/10.1002/ange.201901139 |
Language: | English |
Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
Keywords: | Cytosin, DNA, Epigenetik, Methylierung, Photokäfige |
UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
URI: | https://discovery.ucl.ac.uk/id/eprint/10083335 |
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