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Biological impact of nanodiamond particles – label free, high-resolution methods for nanotoxicity assessment

Khanal, D; Zhang, F; Song, Y; Hau, H; Gautam, A; Yamaguchi, S; Uertz, J; ... Chrzanowski, W; + view all (2019) Biological impact of nanodiamond particles – label free, high-resolution methods for nanotoxicity assessment. Nanotoxicology , 13 (9) pp. 1210-1226. 10.1080/17435390.2019.1650970. Green open access

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Abstract

Current methods for the assessment of nanoparticle safety that are based on 2D cell culture models and fluorescence-based assays show limited sensitivity and they lack biomimicry. Consequently, the health risks associated with the use of many nanoparticles have not yet been established. There is a need to develop in vitro models that mimic physiology more accurately and enable high throughput assessment. There is also a need to set up new assays that offer high sensitivity and are label-free. Here we developed ‘mini-liver’ models using scaffold-free bioprinting and used these models together with label-free nanoscale techniques for the assessment of toxicity of nanodiamond produced by laser-assisted technology. Results showed that NDs induced cytotoxicity in a concentration and exposure-time dependent manner. The loss of cell function was confirmed by increased cell stiffness, decreased cell membrane barrier integrity and reduced cells mobility. We further showed that NDs elevated the production of reactive oxygen species and reduced cell viability. Our approach that combined mini-liver models with label-free high-resolution techniques showed improved sensitivity in toxicity assessment. Notably, this approach allowed for label-free semi-high throughput measurements of nanoparticle-cell interactions, thus could be considered as a complementary approach to currently used methods.

Type: Article
Title: Biological impact of nanodiamond particles – label free, high-resolution methods for nanotoxicity assessment
Open access status: An open access version is available from UCL Discovery
DOI: 10.1080/17435390.2019.1650970
Publisher version: https://doi.org/10.1080/17435390.2019.1650970
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Nanodiamond, nanosafety, 3D liver model, mechanobiology, holotomography
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10082462
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