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Lentiviral Vector Purification Using Nanofiber Ion Exchange Chromatography

Ruscic, J; Perry, C; Mukhopadhyay, T; Takeuchi, Y; Bracewell, DG; (2019) Lentiviral Vector Purification Using Nanofiber Ion Exchange Chromatography. Molecular Therapy: Methods & Clinical Development , 15 pp. 52-62. 10.1016/j.omtm.2019.08.007. Green open access

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Abstract

Lentiviral vectors (LVs) are used in cell and gene therapies due to their ability to transduce both dividing and non-dividing cells while carrying a relatively large genetic payload and providing long-term gene expression via gene integration. Current cultivation methods produce titres of 105 to 107 TU/mL thus it is necessary to concentrate LVs as well as to remove process and product related impurities. In this work we used a packaging cell line WinPac-RD-HV for LV production to simplify upstream processing. A direct capture method based on ion-exchange chromatography and cellulose nanofibers for LV concentration and purification was developed. This novel scalable stationary phase provides a high surface area that is accessible to LV and therefore, has potential for high capacity operation compared to traditional bead-based supports. We were able to concentrate LVs 100-fold while achieving a two-log removal of host cell protein and maintaining up to a 90% yield of functional vector.

Type: Article
Title: Lentiviral Vector Purification Using Nanofiber Ion Exchange Chromatography
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.omtm.2019.08.007
Publisher version: https://doi.org/10.1016/j.omtm.2019.08.007
Language: English
Additional information: © 2019 The Author(s).This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10081764
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