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Development of a 3D Tissue Engineered Skeletal Muscle and Bone Co-culture System

Wragg, NM; Mosqueira, D; Blokpeol-Ferreras, L; Capel, A; Player, DJ; Martin, NRW; Liu, Y; (2020) Development of a 3D Tissue Engineered Skeletal Muscle and Bone Co-culture System. Biotechnology Journal , 15 (1) , Article 1900106. 10.1002/biot.201900106. Green open access

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Abstract

In vitro three-dimensional (3D) tissue engineered (TE) structures have been shown to better represent in vivo tissue morphology and biochemical pathways than monolayer culture, and are less ethically questionable than animal models. However, to create systems with even greater relevance, multiple integrated tissue systems should be recreated in vitro. In the present study, the effects and conditions most suitable for the co-culture of TE skeletal muscle and bone were investigated. High-glucose Dulbecco's Modified Eagle Medium (HG-DMEM) supplemented with 20% foetal bovine serum (FBS) followed by HG-DMEM with 2% horse serum was found to enable proliferation of both C2C12 muscle precursor cells and TE85 human osteosarcoma cells, fusion of C2C12s into myotubes, as well as an up-regulation of RUNX2/CBFa1 in TE85s. Myotube formation was also evident within indirect contact monolayer cultures. Finally, in 3D co-cultures, TE85 collagen/hydroxyapatite constructs had significantly greater expression of RUNX2/CBFa1 and osteocalcin/BGLAP in the presence of collagen-based C2C12 skeletal muscle constructs; however, fusion within these constructs appeared reduced. This work demonstrates the first report of the simultaneous co-culture and differentiation of 3D TE skeletal muscle and bone, and represents a significant step towards a full in vitro 3D musculoskeletal junction model.

Type: Article
Title: Development of a 3D Tissue Engineered Skeletal Muscle and Bone Co-culture System
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/biot.201900106
Publisher version: https://doi.org/10.1002/biot.201900106
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: bone, co-culture, medium compatibility, skeletal muscle, tissue engineering
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/10081515
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