Baud, A;
Heywood, WE;
Little, D;
Gissen, P;
Mills, K;
(2019)
Preparation of iPSCs for Targeted Proteomic Analysis.
Cell-Based Assays Using iPSCs for Drug Development and Testing
, 1994
pp. 131-139.
10.1007/978-1-4939-9477-9_11.
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Abstract
Induced pluripotent stem cells have great potential as a human model system in regenerative medicine, disease modelling and drug screening. However, extensive analysis of iPSC are required before their therapeutic applications. With recent developments in mass spectrometry and proteomics, this technique can become a great alternative to traditional genomic approaches for iPSC analysis. Here, we describe preparation of iPSC for targeted proteomic analysis, and measurement of pluripotency markers allowing for classification into either pluripotent or non-pluripotent cells.
| Type: | Article |
|---|---|
| Title: | Preparation of iPSCs for Targeted Proteomic Analysis |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-9477-9_11 |
| Publisher version: | https://doi.org/10.1007/978-1-4939-9477-9_11 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Induced pluripotent stem cells, pluripotency, proteomics, mass spectrometry |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10080976 |
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