Mazzacuva, F;
Lorvellec, M;
Cilibrizzi, A;
Mills, K;
Clayton, P;
Gissen, P;
(2019)
Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes.
Cell-Based Assays Using iPSCs for Drug Development and Testing
, 1994
pp. 141-147.
10.1007/978-1-4939-9477-9_12.
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Abstract
During the process of differentiation from induced pluripotent stem cells (iPSCs) to hepatocyte-like cells it is crucial to monitor the functionality of cells, in order to avoid an overestimation of the level of maturation. To this end, we propose bile acid profiling as a novel approach which is useful in determining the maturation of hepatocyte-like cells. The main advantages of the method are the simplicity and rapidity of test (i.e., single-step sample preparation followed by 3.5-min analysis), as well as the possibility to localize possible enzyme deficiencies by quantifying the accumulation of specific intermediates involved in the synthetic pathways.
| Type: | Article |
|---|---|
| Title: | Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-4939-9477-9_12 |
| Publisher version: | https://doi.org/10.1007/978-1-4939-9477-9_12 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Bile acids; liposomes; mass spectrometry; liquid chromatography; cholesterol. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10080959 |
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