UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp

Douvaras, P; Dora, NJ; Mort, RL; Lodge, EJ; Hill, RE; West, JD; (2016) Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp. Experimental Eye Research , 149 pp. 26-39. 10.1016/j.exer.2016.05.021. Green open access

[thumbnail of 1-s2.0-S0014483516301245-main.pdf]
Preview
Text
1-s2.0-S0014483516301245-main.pdf - Published Version

Download (3MB) | Preview

Abstract

We investigated the corneal morphology of adult Mp/þ mice, which are heterozygous for the micropinna microphthalmia mutation, and identified several abnormalities, which implied that corneal epithelial maintenance was abnormal. The Mp/þ corneal epithelium was thin, loosely packed and contained goblet cells in older mice. Evidence also suggested that the barrier function was compromised. However, there was no major effect on corneal epithelial cell turnover and mosaic patterns of radial stripes indicated that radial cell movement was normal. Limbal blood vessels formed an abnormally wide limbal vasculature ring, K19-positive cells were distributed more widely than normal and K12 was weakly expressed in the peripheral cornea. This raises the possibilities that the limbal-corneal boundary was poorly defined or the limbus was wider than normal. BrdU label-retaining cell numbers and quantitative clonal analysis suggested that limbal epithelial stem cell numbers were not depleted and might be higher than normal. However, as corneal epithelial homeostasis was abnormal, it is possible that Mp/þ stem cell function was impaired. It has been shown recently that the Mp mutation involves a chromosome 18 inversion that disrupts the Fbn2 and Isoc1 genes and produces an abnormal, truncated fibrillin-2MP protein. This abnormal protein accumulates in the endoplasmic reticulum (ER) of cells that normally express Fbn2 and causes ER stress. It was also shown that Fbn2 is expressed in the corneal stroma but not the corneal epithelium, suggesting that the presence of truncated fibrillin-2MP protein in the corneal stroma disrupts corneal epithelial homeostasis in Mp/þ mice.

Type: Article
Title: Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.exer.2016.05.021
Publisher version: https://doi.org/10.1016/j.exer.2016.05.021
Language: English
Additional information: Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Corneal epithelium, Corneal homeostasis, Limbal epithelial stem cells, Limbus, Micropinna microphthalmia mutant, Mouse, Mp mutant
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10080725
Downloads since deposit
101Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item