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Virus lasers for biological detection

Hales, JE; Matmon, G; Dalby, PA; Ward, JM; Aeppli, G; (2019) Virus lasers for biological detection. Nature Communications , 10 (1) , Article 3594. 10.1038/s41467-019-11604-z. Green open access

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Abstract

The selective amplification of DNA in the polymerase chain reaction is used to exponentially increase the signal in molecular diagnostics for nucleic acids, but there are no analogous techniques for signal enhancement in clinical tests for proteins or cells. Instead, the signal from affinity-based measurements of these biomolecules depends linearly on the probe concentration. Substituting antibody-based probes tagged for fluorescent quantification with lasing detection probes would create a new platform for biomarker quantification based on optical rather than enzymatic amplification. Here, we construct a virus laser which bridges synthetic biology and laser physics, and demonstrate virus-lasing probes for biosensing. Our virus-lasing probes display an unprecedented > 10,000 times increase in signal from only a 50% increase in probe concentration, using fluorimeter-compatible optics, and can detect biomolecules at sub-100 fmol mL-1 concentrations.

Type: Article
Title: Virus lasers for biological detection
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41467-019-11604-z
Publisher version: https://doi.org/10.1038/s41467-019-11604-z
Language: English
Additional information: © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Biosensors, Characterization and analytical techniques, Lasers, LEDs and light sources
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10080517
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