Bage, T;
Edymann, T;
Metcalfe, AD;
Dheansa, B;
Mbundi, L;
(2019)
Ex vivo culture of keratinocytes on papillary and reticular dermal layers remodels skin explants differently: towards improved wound care.
Archives of Dermatological Research
, 311
pp. 647-652.
10.1007/s00403-019-01941-w.
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Ex vivo culture of keratinocytes on papillary and reticular dermal layers remodels skin explants differently.pdf - Published Version Download (1MB) | Preview |
Abstract
In this study, we characterised the efect that seeding keratinocytes on the papillary and reticular dermis had on the extracellular matrix and tissue integrity ex vivo. Human skin explants from consented patients (n=6) undergoing routine surgery were cultured at a liquid–air interface, dermal-side up, and autologous keratinocytes seeded on the exposed papillary or reticular layer. After 7–21 days, histological and immunohistochemical evaluation of the morphology and extracellular matrix was performed. While the dermis remained robust in all explants cultures, keratinocytes seeded on the papillary layer showed less tissue infltration and remodelling and formed clusters across the tissue. In contrast, keratinocytes seeded on the reticular layer infltrated the tissue homogenously with an intact single-cell-layer surface coverage and structural changes characterised by increased deposition of ground substance, glycosaminoglycans, and collagen VII in 14 days. In addition, while the papillary section showed more new laminin deposition by 14 days than the reticular section, the latter expressed more connexin 43. These diferences in re-epithelialisation and extracellular matrix characteristics suggest that wound depth and graft thickness may play a key role in wound healing and indicate that ECM characteristics should be factored in when designing biomaterials for wound applications and in the selection of recipient sites when using cells for grafting.
| Type: | Article |
|---|---|
| Title: | Ex vivo culture of keratinocytes on papillary and reticular dermal layers remodels skin explants differently: towards improved wound care |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s00403-019-01941-w |
| Publisher version: | https://doi.org/10.1007/s00403-019-01941-w |
| Language: | English |
| Additional information: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| Keywords: | Papillary · Reticular · Keratinocytes · ECM |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10079557 |
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