Wilke, C;
Pujol-Calderón, F;
Barro, C;
Stransky, E;
Blennow, K;
Michalak, Z;
Deuschle, C;
... Synofzik, M; + view all
(2019)
Correlations between serum and CSF pNfH levels in ALS, FTD and controls: a comparison of three analytical approaches.
Clinical Chemistry and Laboratory Medicine
, 57
(10)
pp. 1556-1564.
10.1515/cclm-2019-0015.
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Zetterberg [Clinical Chemistry and Laboratory Medicine (CCLM)] Correlations between serum and CSF pNfH levels in ALS FTD and controls a comparison of three analytical approac.pdf - Published Version Download (782kB) | Preview |
Abstract
Background: Phosphorylated neurofilament heavy (pNfH), a neuronal cytoskeleton protein, might provide a promising blood biomarker of neuronal damage in neurodegenerative diseases (NDDs). The best analytical approaches to measure pNfH levels and whether serum levels correlate with cerebrospinal fluid (CSF) levels in NDDs remain to be determined. / Methods: We here compared analytical sensitivity and reliability of three novel analytical approaches (homebrew Simoa, commercial Simoa and ELISA) for quantifying pNfH in both CSF and serum in samples of amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD) and control subjects. / Results: While all three assays showed highly correlated CSF measurements, Simoa assays also yielded high between-assay correlations for serum measurements (ϱ = 0.95). Serum levels also correlated strongly with CSF levels for Simoa-based measurements (both ϱ = 0.62). All three assays allowed distinguishing ALS from controls by increased CSF pNfH levels, and Simoa assays also by increased serum pNfH levels. pNfH levels were also increased in FTD. / Conclusions: pNfH concentrations in CSF and, if measured by Simoa assays, in blood might provide a sensitive and reliable biomarker of neuronal damage, with good between-assay correlations. Serum pNfH levels measured by Simoa assays closely reflect CSF levels, rendering serum pNfH an easily accessible blood biomarker of neuronal damage in NDDs.
| Type: | Article |
|---|---|
| Title: | Correlations between serum and CSF pNfH levels in ALS, FTD and controls: a comparison of three analytical approaches |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1515/cclm-2019-0015 |
| Publisher version: | https://doi.org/10.1515/cclm-2019-0015 |
| Language: | English |
| Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | amyotrophic lateral sclerosis (ALS), cerebrospinal fluid (CSF), frontotemporal dementia (FTD), phosphorylated neurofilament heavy chain (pNfH), serum, single molecule array (Simoa) |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10079463 |
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