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Osteochondral Tissue Co-Culture: An In Vitro and In Silico Approach

Xue, R; Chung, B; Tamaddon, M; Carr, J; Liu, C; Cartmell, S; (2019) Osteochondral Tissue Co-Culture: An In Vitro and In Silico Approach. Biotechnology and Bioengineering , 116 (11) pp. 3112-3123. 10.1002/bit.27127. Green open access

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Abstract

Osteochondral tissue engineering aims to regenerate functional tissue mimicking physiological properties of injured cartilage and its subchondral bone. Given the distinct structural and biochemical difference between bone and cartilage, bi-layered scaffolds and bioreactors are commonly employed. We present an osteochondral culture system which co-cultured ATDC5 and MC3T3-E1 cells on an additive manufactured bi-layered scaffold in a dual-chamber perfusion bioreactor. Also, finite element models (FEM) based on the micro-computed tomography (μCT) image of the manufactured scaffold as well as on the computer aided design (CAD) were constructed; the microenvironment inside the two FEM was studied and compared. In vitro results showed that the co-culture system supported osteochondral tissue growth in terms of cell viability, proliferation, distribution and attachment. In silico results showed that the CAD and the actual manufactured scaffold had significant differences in the flow velocity, differentiation media mixing in the bioreactor and fluid-induced shear stress (FSS) experienced by the cells. This system was shown to have desired microenvironment for ostechondral tissue engineering and it can potentially be used as an inexpensive tool for testing newly developed pharmaceutical products for osteochondral defects. This article is protected by copyright. All rights reserved.

Type: Article
Title: Osteochondral Tissue Co-Culture: An In Vitro and In Silico Approach
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/bit.27127
Publisher version: https://doi.org/10.1002/bit.27127
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Additive tissue engineering, bi-layered scaffold, co-culture, finite element analysis, osteochondral tissue engineering
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/10078972
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