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Metagenomics in ophthalmology: Hypothesis or real prospective?

Gallon, P; Parekh, M; Ferrari, S; Fasolo, A; Ponzin, D; Borroni, D; (2019) Metagenomics in ophthalmology: Hypothesis or real prospective? [Review]. Biotechnology Reports , 23 , Article e00355. 10.1016/j.btre.2019.e00355. (In press). Green open access

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Abstract

Metagenomic analysis was originally associated with the studies of genetic material from environmental samples. But, with the advent of the Human Microbiome Project, it has now been applied in clinical practices. The ocular surface (OS) is the most exposed part of the eye, colonized by several microbial communities (both, OS and environmental) that contribute to the maintenance of the physiological state. Limited knowledge has been acquired on these microbes due to the limitations of conventional diagnostic methods. Emerging fields of research are focusing on Next Generation Sequencing (NGS) technologies to obtain reliable information on the OS microbiome. Currently only pre-specified pathogens can be detected by conventional culture-based techniques or Polymerase Chain Reaction (PCR), but there are conditions to state whether metagenomics could revolutionize the diagnosis of ocular diseases. The aim of this review is to provide an updated overview of the studies involving NGS technology for OS microbiome

Type: Article
Title: Metagenomics in ophthalmology: Hypothesis or real prospective?
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.btre.2019.e00355
Publisher version: https://doi.org/10.1016/j.btre.2019.e00355
Language: English
Additional information: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords: Metagenomics, Ocular infection, NGS (next generation sequencing), Ocular surface, Microbiome
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10078536
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