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A thermal ink-jet printing approach for evaluating susceptibility of bacteria to antibiotics

Dodoo, CC; Alomari, M; Basit, AW; Stapleton, P; Gaisford, S; (2019) A thermal ink-jet printing approach for evaluating susceptibility of bacteria to antibiotics. Journal of Microbiological Methods , Article 105660. 10.1016/j.mimet.2019.105660. Green open access

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Abstract

An inexpensive method for determining minimum inhibitory concentrations (MIC) using ink-jet printing to deposit drug solutions and bacterial suspensions onto agar was developed. Substrate concentrations were varied using a "Y-value", whereby a series of rectangles with the same width and colour but different heights were printed within a fixed unit area. Prior to MIC determination, the printer cartridges used were calibrated using Fast Green dye. The impact of thermal ink-jet printing on bacterial viability was assessed by colony counting and found not to be deleterious. MIC determinations were conducted by printing varying concentrations of the antibiotics onto agar-coated glass slides then printing a thin even film of a known bacterial density of Lactobacillus acidophilus. Broth microdilution was performed simultaneously to validate the results. Slides and well plates were then incubated anaerobically for 48 h. The MIC values obtained for the antibiotics used were within a permissible range for comparison.

Type: Article
Title: A thermal ink-jet printing approach for evaluating susceptibility of bacteria to antibiotics
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.mimet.2019.105660
Publisher version: https://doi.org/10.1016/j.mimet.2019.105660
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Antimicrobial resistance, Minimum inhibitory concentration, Thermal ink-jet printing, Y-value
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery.ucl.ac.uk/id/eprint/10078242
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