UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

The neuropeptide Galanin is required for homeostatic rebound sleep following increased neuronal activity

Reichert, S; Pavón Arocas, O; Rihel, J; (2019) The neuropeptide Galanin is required for homeostatic rebound sleep following increased neuronal activity. Neuron , 104 (2) 370-384.e5. 10.1016/j.neuron.2019.08.010.

[img] Text
NEURON-D-18-01816_R2 (1).pdf - Accepted version
Access restricted to UCL open access staff until 17 September 2020.

Download (210MB)

Abstract

Sleep pressure homeostatically increases during wake and dissipates during sleep, but the molecular signals and neuronal substrates that measure homeostatic sleep pressure remain poorly understood. We present a pharmacological assay in larval zebrafish that generates acute, short-term increases in wakefulness followed by sustained rebound sleep after washout. The intensity of global neuronal activity during drug-induced wakefulness predicted the amount of subsequent rebound sleep. Whole-brain mapping with the neuronal activity marker phosphorylated extracellular signal–regulated kinase (pERK) identified preoptic Galanin (Galn)-expressing neurons as selectively active during rebound sleep, and the relative induction of galn transcripts was predictive of total rebound sleep time. Galn is required for sleep homeostasis, as galn mutants almost completely lacked rebound sleep following both pharmacologically-induced neuronal activity and physical sleep deprivation. These results suggest that Galn plays a key role in responding to sleep pressure signals derived from neuronal activity and functions as an output arm of the vertebrate sleep homeostat

Type: Article
Title: The neuropeptide Galanin is required for homeostatic rebound sleep following increased neuronal activity
DOI: 10.1016/j.neuron.2019.08.010
Publisher version: https://doi.org/10.1016/j.neuron.2019.08.010
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: sleep, zebrafish, homeostasis, galanin, hypothalamus
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10077804
Downloads since deposit
1Download
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item