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In situ structure determination by subtomogram averaging

Castaño-Díez, D; Zanetti, G; (2019) In situ structure determination by subtomogram averaging. Current Opinion in Structural Biology , 58 pp. 68-75. 10.1016/j.sbi.2019.05.011. Green open access

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Abstract

Cryo-tomography and subtomogram averaging are increasingly popular techniques for structural determination of macromolecular complexes in situ. They have the potential to achieve high-resolution views of native complexes, together with the details of their location relative to interacting molecules. The subtomogram averaging (StA) pipelines are well-established, with current developments aiming to optimise each step by reducing manual intervention and user decisions, following similar trends in single-particle approaches that have dramatically increased their popularity. Here, we review the main steps of typical StA workflows. We focus on considerations arising from the fact that the objects of study are embedded within unique crowded environments, and we emphasise those steps where careful decisions need to be made by the user.

Type: Article
Title: In situ structure determination by subtomogram averaging
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.sbi.2019.05.011
Publisher version: https://doi.org/10.1016/j.sbi.2019.05.011
Language: English
Additional information: © 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10077580
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