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Improving product specificity of whole-cell alkane oxidation in non-conventional media: a multivariate analysis approach

Kolmar, JF; Thum, O; Baganz, F; (2019) Improving product specificity of whole-cell alkane oxidation in non-conventional media: a multivariate analysis approach. Biotechnology Journal , 14 (10) , Article 1800581. 10.1002/biot.201800581. Green open access

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Abstract

Two-liquid phase reaction media have long been used in bioconversions to supply or remove hydrophobic organic reaction substrates and products to reduce inhibitory and toxic effects on biocatalysts. In case of the terminal oxyfunctionalisation of linear alkanes by the AlkBGT monooxygenase the excess alkane substrate is often used as a second phase to extract the alcohol, aldehyde and acid products. However, the selection of other carrier phases or surfactants is complex due to the large amount of parameters that are involved, such as: biocompatibility, substrate bioavailability and product extraction selectivity. This study combines systematic high-throughput screening with chemometrics to correlate physicochemical parameters of a range of co-solvents to product specificity and yield using a multivariate regression model. Partial least square regression showed that the defining factor for product specificity are the solubility properties of reaction substrate and product in the co-solvent, as measured by Hansen solubility parameters. Thus the polarity of co-solvents determines the accumulation of either alcohol or acid products. Whereas usually the acid product accumulates during the reaction, by choosing a more polar co-solvent the 1-alcohol product can be accumulated. Especially with Tergitol as co-solvent, a 3.2 fold improvement in 1-octanol yield to 18.3 mmol l-1 was achieved relative to the control reaction without co-solvents. This article is protected by copyright. All rights reserved.

Type: Article
Title: Improving product specificity of whole-cell alkane oxidation in non-conventional media: a multivariate analysis approach
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/biot.201800581
Publisher version: https://doi.org/10.1002/biot.201800581
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Alkane monooxygenase, High-throughput, Multivariate data analysis, Non-conventional media, Whole-cell bio-oxidation
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10077555
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