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In Vivo and In Vitro Quantitative Analysis of Neural Crest Cell Migration

Barriga, EH; Shellard, A; Mayor, R; (2019) In Vivo and In Vitro Quantitative Analysis of Neural Crest Cell Migration. Methods in Molecular Biology (MIMA) , 1976 pp. 135-152. 10.1007/978-1-4939-9412-0_11.

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Abstract

The neural crest is an embryonic cell population induced at the border of the neural plate from where it delaminates and migrates long distances across the embryo. Due to its extraordinary migratory capabilities, the neural crest has become a powerful system to study cellular and molecular aspects of collective and single cell migration both in vivo and in vitro. Here we provide detailed protocols used to perform quantitative analysis of molecular and cellular aspects of Xenopus laevis neural crest cell migration, both in vivo and in vitro.

Type: Article
Title: In Vivo and In Vitro Quantitative Analysis of Neural Crest Cell Migration
DOI: 10.1007/978-1-4939-9412-0_11
Publisher version: https://doi.org/10.1007/978-1-4939-9412-0_11
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Cell dispersion, Cell migration, Delaunay-Voronoi triangulation, Grafts, In situ hybridization, Neural crest cells, Neural crest dissection, Quantitative migration, Xenopus laevis
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10077226
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