Hirani, N;
Illukkumbura, R;
Bland, T;
Mathonnet, G;
Suhner, D;
Reymann, A-C;
Goehring, NW;
(2019)
Anterior-enriched filopodia create appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes.
Journal of Cell Science
, 132
(14)
, Article jcs230714. 10.1242/jcs.230714.
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Abstract
The association of molecules within membrane microdomains is critical for the intracellular organization of cells. During polarization of the C. elegans zygote, both polarity proteins and actomyosin regulators associate within dynamic membrane-associated foci. Recently, a novel class of asymmetric PIP2 membrane-associated structures was described, suggesting that PIP2 domains could constitute signaling hubs to promote cell polarization and actin nucleation. Here we probe the nature of these domains using a variety of membrane- and actin cortex-associated probes. These data demonstrate that these domains are filopodia, which are stimulated transiently during polarity establishment and accumulate in the zygote anterior. The resulting membrane protrusions create local membrane topology that quantitatively accounts for observed local increases in fluorescence signal of membrane-associated molecules, suggesting molecules are not selectively enriched in these domains relative to bulk membrane and that the PIP2 pool as revealed by PHPLCδ1 simply reflects plasma membrane localization. Given the ubiquity of 3D membrane structures in cells, including filopodia, microvilli, and membrane folds, similar caveats are likely to apply to analysis of membrane-associated molecules in a broad range of systems.
Type: | Article |
---|---|
Title: | Anterior-enriched filopodia create appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes |
Location: | England |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1242/jcs.230714 |
Publisher version: | https://doi.org/10.1242/jcs.230714 |
Language: | English |
Additional information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
URI: | https://discovery.ucl.ac.uk/id/eprint/10077093 |
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