Pritchard, RH;
Zhukov, AA;
Fullerton, JN;
Want, AJ;
Hussain, F;
la Cour, MF;
Bashtanov, ME;
... Rogers, SS; + view all
(2019)
Cell sorting actuated by a microfluidic inertial vortex.
Lab on a Chip
10.1039/c9lc00120d.
(In press).
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Abstract
The sorting of specific cell populations is an established tool in biological research, with new applications demanding greater cell throughput, sterility and elimination of cross-contamination. Here we report ‘vortex-actuated cell sorting’ (VACS), a new technique that deflects cells individually, via the generation of a transient microfluidic vortex by a thermal vapour bubble: a novel mechanism, which is able to sort cells based on fluorescently-labelled molecular markers. Using in silico simulation and experiments on beads, an immortal cell line and human peripheral blood mononuclear cells (PBMCs), we demonstrate high-purity and high-recovery sorting with input rates up to 10^{4} cells per s and switching speeds comparable to existing techniques (>40 kHz). A tiny footprint (1 × 0.25 mm) affords miniaturization and the potential to achieve multiplexing: a crucial step in increasing processing rate. Simple construction using biocompatible materials potentially minimizes cost of fabrication and permits single-use sterile cartridges. We believe VACS potentially enables parallel sorting at throughputs relevant to cell therapy, liquid biopsy and phenotypic screening.
| Type: | Article |
|---|---|
| Title: | Cell sorting actuated by a microfluidic inertial vortex |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/c9lc00120d |
| Publisher version: | http://dx.doi.org/10.1039/c9lc00120d |
| Language: | English |
| Additional information: | © The Royal Society of Chemistry 2019. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10076717 |
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