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Phenotyping Microarrays for the Characterization of Environmental Microorganisms

Low-Décarie, E; Lofano, A; Samani, P; (2015) Phenotyping Microarrays for the Characterization of Environmental Microorganisms. In: Hydrocarbon and Lipid Microbiology Protocols. (pp. 167-181). Springer: Cham, Switzerland.

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Abstract

Culture-based methods for the characterization of microorganisms remain essential to advances in microbiology. Phenotyping arrays and microplates in which each well represents a different selective growth environment are important tools (1) in the identification of microbial isolates, (2) in the characterization of the phenotypic fingerprint of microbial communities, (3) for linking specific functions with specific organisms or genes, and (4) for the identification of evolutionary trade-offs in the establishment of phenotypes. The use of phenotyping arrays in the study of hydrocarbon and lipid degradation by microbial isolates or communities is an emerging application. The application of phenotyping arrays requires careful selection of substrates, growth medium, and dyes and consideration of the intrinsic limitations of the approach. The use of phenotyping arrays leads to the production of large amounts of data, which require specific approaches for summarization and analysis. Liquid handling automation will increase the feasibility of custom phenotyping arrays that include hydrocarbons and lipids.

Type: Book chapter
Title: Phenotyping Microarrays for the Characterization of Environmental Microorganisms
ISBN: 3662491400
ISBN-13: 9783662491409
DOI: 10.1007/8623_2015_106
Publisher version: https://doi.org/10.1007/8623_2015_106
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Biodegradation, Biolog™, Culturomics, Ecotype, High throughput, Microtiter, Phenomics, Phenotyping microarray (PM), Substrate
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10075981
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